Effect of transglutaminase-gene disruption in Streptomyces hygroscopicus on cell differentiation].

Kangkang Chen, Song Liu, Xiaohui Ju, Tengbo Ma, Dongxu Zhang, Guocheng Du, Jian Chen

State Key Laboratory of Food Science and Technology, Ministry of Education, Jiangnan University, Wuxi 214122, China. jnckkang@126.com

Wei sheng wu xue bao = Acta microbiologica Sinica 2010 Dec

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To provide theoretical basis for understanding the physiological function of transglutaminase in Streptomyces, which would benefit for improving the production of this enzyme, transglutaminase gene was disrupted in Streptomyces hygroscopicus. First, the interruption plasmid pKC1139-TG1 was constructed by inserting a segment of transglutaminase gene in the temperature-sensitive vector pKC1139. Second, pKC1139-TG1 was transformed into S. hygroscopicus protoplast and the interruption plasmid was inserted into the chromosome by homogenous recombination. Third, the strain with disrupted transglutaminase gene was screened by apramycin resistance and named as S. h-delta TG. Compared with the parent strain, S. h-delta TG was able to grow as substrate mycelium, but not able to form aerial hyphae. Transglutaminase may be involved in the aerial hyphae formation of S. hygroscopicus.

Citation

Kangkang Chen, Song Liu, Xiaohui Ju, Tengbo Ma, Dongxu Zhang, Guocheng Du, Jian Chen. Effect of transglutaminase-gene disruption in Streptomyces hygroscopicus on cell differentiation]. Wei sheng wu xue bao = Acta microbiologica Sinica. 2010 Dec;50(12):1626-32