Development of a stable isotope dilution assay for tenuazonic acid.

Stefan Asam, Yang Liu, Katharina Konitzer, Michael Rychlik

Technische Universität München, Lehrstuhl für Analytische Lebensmittelchemie, Alte Akademie 10, D-85354 Freising, Germany.

Journal of agricultural and food chemistry 2011 Apr 13

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A stable isotope dilution assay (SIDA) for the Alternaria mycotoxin tenuazonic acid was developed. Therefore, [(13)C(6),(15)N]-tenuazonic acid was synthesized from [(13)C(6),(15)N]-isoleucine by Dieckmann intramolecular cyclization after acetoacetylation with diketene. The synthesized [(13)C(6),(15)N]-tenuazonic acid was used as the internal standard for determination of tenuazonic acid in tomato products by liquid chromatography tandem mass spectrometry after derivatization with 2,4-dinitrophenylhydrazine. Method validation revealed a limit of detection of 0.1 μg/kg and a limit of quantitation of 0.3 μg/kg. Recovery was close to 100% in the range of 3-300 μg/kg. Determination of tenuazonic acid in two samples of different tomato ketchups (naturally contaminated) was achieved with a coefficient of variation of 2.3% and 4.7%. Different tomato products (n = 16) were analyzed for their content of tenuazonic acid using the developed SIDA. Values were between 15 and 195 μg/kg (tomato ketchup, n = 9), 363 and 909 μg/kg (tomato paste, n = 2), and 8 and 247 μg/kg (pureed tomatoes and comparable products, n = 5).

Citation

Stefan Asam, Yang Liu, Katharina Konitzer, Michael Rychlik. Development of a stable isotope dilution assay for tenuazonic acid. Journal of agricultural and food chemistry. 2011 Apr 13;59(7):2980-7