Measurement of type I interferon production.

Rosalind E Seeds, Joanna L Miller

Sir William Dunn School of Pathology, University of Oxford, United Kingdom.

Current protocols in immunology / edited by John E. Coligan ... [et al.] 2011 Feb

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The Basic Protocol in this unit describes measurement of murine interferon (IFN)α/β by intracellular staining for these cytokines and detection by flow cytometry. Alternate protocols detail an enzyme-linked immunoabsorbent assay (ELISA) for IFNα and a biological assay to measure IFN. The FACS assay allows measurement of IFNα/β production by defined cell populations, while ELISA measures secreted IFNα. The bioassay measures functional antiviral activity and is the most sensitive of the assays discussed. These assays therefore provide complementary methods to assess IFN production by murine cells. © 2011 by John Wiley & Sons, Inc.

Citation

Rosalind E Seeds, Joanna L Miller. Measurement of type I interferon production. Current protocols in immunology / edited by John E. Coligan ... [et al.]. 2011 Feb;Chapter 14:Unit14.21.1-11