Design of recombinant antibody microarrays for membrane protein profiling of cell lysates and tissue extracts.

Linda Dexlin-Mellby, Anna Sandström, Linn Antberg, Jenny Gunnarsson, Stefan R Hansson, Carl A K Borrebaeck, Christer Wingren

Department of Immunotechnology, BMC D13, Lund University, Lund, Sweden.

Proteomics 2011 Apr

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Generating global protein expression profiles, including also membrane proteins, will be crucial for our understanding of biological processes in health and disease. In this study, we have expanded our antibody microarray technology platform and designed the first human recombinant antibody microarray for membrane proteins targeting crude cell lysates and tissue extracts. We have optimized all key technological parameters and successfully developed a setup for extracting, labeling and analyzing non-fractionated membrane proteomes under non-denaturing conditions. Finally, the platform was also extended and shown to be compatible with simultaneous profiling of both membrane proteins and water-soluble proteins. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Linda Dexlin-Mellby, Anna Sandström, Linn Antberg, Jenny Gunnarsson, Stefan R Hansson, Carl A K Borrebaeck, Christer Wingren. Design of recombinant antibody microarrays for membrane protein profiling of cell lysates and tissue extracts. Proteomics. 2011 Apr;11(8):1550-4