Effects of granule-bound starch synthase I-defective mutation on the morphology and structure of pyrenoidal starch in Chlamydomonas.

Asako Izumo, Shoko Fujiwara, Toshihiro Sakurai, Steven G Ball, Yoshimi Ishii, Hikaru Ono, Mayumi Yoshida, Naoko Fujita, Yasunori Nakamura, Alain Buléon, Mikio Tsuzuki

School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Horinouchi, Hachioji, Tokyo 192-0392, Japan.

Plant science : an international journal of experimental plant biology 2011 Feb

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Lowering of the CO₂ concentration in the environment induces development of a pyrenoidal starch sheath, as well as that of pyrenoid and CO₂-concentrating mechanisms, in many microalgae. In the green algae Chlamydomonas and Chlorella, activity of granule-bound starch synthase (GBSS) concomitantly increases under these conditions. In this study, effects of the GBSS-defective mutation (sta2) on the development of pyrenoidal starch were investigated in Chlamydomonas. Stroma starch- and pyrenoid starch-enriched samples were obtained from log-phase cells grown with air containing 5% CO₂ (high-CO₂ conditions favouring stromal starch synthesis) and from those transferred to low-CO₂ conditions (air level, 0.04% CO₂, favouring pyrenoidal starch synthesis) for 6h, respectively. In the wild type, total starch content per culture volume did not increase during the low-CO₂ conditions, in spite of the development of pyrenoidal starch, suggesting that degradation of some part of stroma starch and synthesis of pyrenoid starch simultaneously occur under these conditions. Even in the GBSS-deficient mutants, pyrenoid and pyrenoid starch enlarged after lowering of the CO₂ concentration. However, the morphology of the pyrenoid starch was thinner and more fragile than the wild type, suggesting that GBSS does affect the morphology of pyrenoidal starch. Surprisingly normal GBSS activity is shown to be required to obtain the high A-type crystallinity levels that we now report for pyrenoidal starch. A model is presented explaining how GBSS-induced starch granule fusion may facilitate the formation of the pyrenoidal starch sheath. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

Citation

Asako Izumo, Shoko Fujiwara, Toshihiro Sakurai, Steven G Ball, Yoshimi Ishii, Hikaru Ono, Mayumi Yoshida, Naoko Fujita, Yasunori Nakamura, Alain Buléon, Mikio Tsuzuki. Effects of granule-bound starch synthase I-defective mutation on the morphology and structure of pyrenoidal starch in Chlamydomonas. Plant science : an international journal of experimental plant biology. 2011 Feb;180(2):238-45