Gold nanoparticles deposited capillaries for in-capillary microextraction capillary zone electrophoresis of monohydroxy-polycyclic aromatic hydrocarbons.

Huiyong Wang, Gaston Knobel, Walter B Wilson, Korina Calimag-Williams, Andres D Campiglia

Department of Chemistry, University of Central Florida, Orlando, FL 32816-2366, USA.

Electrophoresis 2011 Mar

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This article presents the first application of gold nanoparticles deposited capillaries as pre-concentration devices for in-capillary microextraction CZE and their use for the analysis of monohydroxy-polycyclic aromatic hydrocarbons in synthetic urine samples. The successful separation of 1-hydroxypyrene, 9-hydroxyphenanthrene, 3-hydroxybenzo[a]pyrene (3-OHbap), 4-hydroxybenzo[a]pyrene and 5-hydroxybenzo[a]pyrene under a single set of electrophoretic conditions is demonstrated as well as the feasibility to obtain competitive ultraviolet absorption LOD with commercial instrumentation. Enrichment factors ranging from 87 (9-OHphe) to 100 (3-OHbap) made it possible to obtain LOD ranging from 9 ng/mL (9-OHphe and 3-OHbap) to 14 ng/mL (4-hydroxybenzo[a]pyrene). Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Huiyong Wang, Gaston Knobel, Walter B Wilson, Korina Calimag-Williams, Andres D Campiglia. Gold nanoparticles deposited capillaries for in-capillary microextraction capillary zone electrophoresis of monohydroxy-polycyclic aromatic hydrocarbons. Electrophoresis. 2011 Mar;32(6-7):720-7