A primer set to determine sex in the small Indian mongoose, Herpestes auropunctatus.

C Murata, G Ogura, A Kuroiwa

Graduate School of Life Science, Hokkaido University, Kita 10 Nishi 8, Kita-ku, Sapporo, Hokkaido, Japan.

Molecular ecology resources 2011 Mar

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To enable the accurate sexing of individuals of introduced populations of the small Indian mongoose, Herpestes auropunctatus, we designed a primer set for the amplification of the sex-specific fragments EIF2S3Y and EIF2S3X. Using this primer set, the expected amplification products were obtained for all samples of genomic DNA tested: males yielded two bands and females a single band. Sequencing of each PCR product confirmed that the 769-bp fragment amplified from DNA samples of both sexes was derived from EIF2S3X, whereas the 546-bp fragment amplified only from male DNA samples was derived from EIF2S3Y. The results indicated that this primer set is useful for sex identification in this species. © 2010 Blackwell Publishing Ltd.

Citation

C Murata, G Ogura, A Kuroiwa. A primer set to determine sex in the small Indian mongoose, Herpestes auropunctatus. Molecular ecology resources. 2011 Mar;11(2):386-8