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This paper quantificationally probes into time-varying migration processes of moving neutralization boundary (MNB) on immobilized pH gradient (IPG) strip in ammonia-rehydration buffers. The time-varying migration processes are determined by both time-varying dissociation equilibria of ammonia and position-varying pH environments formed by immobilized carrier ampholytes (CAs) on the IPG strip. Thus, the local dissociation equilibria of ammonia and the position-varying pH are introduced into the recursion equation of position of MNB migrations. The theoretical position-time curves and the velocity-time curves of MNB migrations obtained by the recursion approach were satisfactorily validated by a series of images of boundary migrations from the IPG-MNB experiments by using rehydration buffers with different ammonia concentrations on pH 3-6 IPG strips. The results achieved herein have significant evidence to a quantificational understanding of the mechanism of MNB and IEF. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Heng Liang, Liang-Fei OuYang, Qian Liu, Lin Zhang, Li-Jing Tian, Yang Chen. Time-varying migration process of moving neutralization boundary on the immobilized pH gradient strip in the weak-base rehydration buffer. Journal of separation science. 2011 May;34(10):1212-9

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PMID: 21495187

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