Kinetic resolution of 3-fluoroalanine using a fusion protein of D-amino acid oxidase with Vitroscilla hemoglobin.

Young-Man Seo, Yong-Ho Khang, Hyungdon Yun

School of Biotechnology, Yeungnam University, Japan.

Bioscience, biotechnology, and biochemistry 2011

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In this study, a fusion protein (VHb-DAAO) of D-amino acid oxidase (DAAO) with Vitreoscilla hemoglobin (VHb) was functionally expressed in Escherichia coli and purified. The k(cat) value VHb-DAAO (47.1 s⁻¹) towards rac-3-flouroalanine was about 2-fold higher than that of DAAO (21.9 s⁻¹). rac-3-Flouroalanine (500 mM) was kinetically resolved into (R)-3-fluoroalanine with high enatiomeric excess (>99%) by VHb-DAAO with about 52% conversion.

Citation

Young-Man Seo, Yong-Ho Khang, Hyungdon Yun. Kinetic resolution of 3-fluoroalanine using a fusion protein of D-amino acid oxidase with Vitroscilla hemoglobin. Bioscience, biotechnology, and biochemistry. 2011;75(4):820-2