Bioconversion of d-xylose to d-xylonate with Kluyveromyces lactis.

Yvonne Nygård, Mervi H Toivari, Merja Penttilä, Laura Ruohonen, Marilyn G Wiebe

VTT, Technical Research Centre of Finland, VTT Espoo, Finland. yvonne.nygard@vtt.fi

Metabolic engineering 2011 Jul

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d-Xylonate was produced from d-xylose using Kluyveromyces lactis strains which expressed the gene for NADP(+)-dependent d-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19 ± 2g d-xylonatel(-1) was produced when K. lactis expressing xyd1 was grown on 10.5 gd-galactosel(-1) and 40 g d-xylosel(-1). Intracellular accumulation of d-xylonate (up to ∼70 mg [gbiomass](-1)) was observed. d-Xylose was metabolised to d-xylonate, xylitol and biomass. Oxygen could be reduced to 6mmolO(2)l(-1)h(-1) without loss in titre or production rate, but metabolism of d-xylose and xylitol were more efficient when 12 mmolO(2)l(-1)h(-1) were provided. d-Xylose uptake was not affected by deletion of either the d-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1ΔXYL1 did not produce extracellular xylitol and produced more d-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1ΔXYL2 produced high concentrations of xylitol and significantly less d-xylonate than the xyd1 strain with the endogenous XYL2. Copyright © 2011 Elsevier Inc. All rights reserved.

Citation

Yvonne Nygård, Mervi H Toivari, Merja Penttilä, Laura Ruohonen, Marilyn G Wiebe. Bioconversion of d-xylose to d-xylonate with Kluyveromyces lactis. Metabolic engineering. 2011 Jul;13(4):383-91