In vitro RISC cleavage assay.

RNA interference (RNAi) is a widely used tool for the analysis of gene expression. In this process, short-interfering RNAs (siRNAs) guide the RNA-induced silencing complex (RISC) to complementary target RNA molecules, which are sequence-specifically cleaved by the RISC. In vitro cleavage assays have proved to be powerful tools for the characterization of the RNAi pathway in many different organisms. Therefore, this chapter provides a detailed protocol for in vitro RISC assays.

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Julia Stoehr, Gunter Meister. In vitro RISC cleavage assay. Methods in molecular biology (Clifton, N.J.). 2011;725:77-90

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PMID: 21528448

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