Tomoko Kawamata, Yukihide Tomari
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan.
Methods in molecular biology (Clifton, N.J.) 2011Small-interfering RNAs (siRNAs) and microRNAs (miRNAs) regulate expression of their target mRNAs via the RNA-induced silencing complex (RISC). A core component of RISC is the Argonaute (Ago) protein, which dictates the RISC function. In Drosophila, miRNAs and siRNAs are generally loaded into Ago1-containing RISC (Ago1-RISC) and Ago2-containing RISC (Ago2-RISC), respectively. We developed a native agarose gel system to directly detect Ago1-RISC, Ago2-RISC, and their precursor complexes. Methods presented here will provide powerful tools to biochemically dissect the RISC assembly pathways.
Tomoko Kawamata, Yukihide Tomari. Native gel analysis for RISC assembly. Methods in molecular biology (Clifton, N.J.). 2011;725:91-105
PMID: 21528449
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