Angiotensin II as a factor modulating protein tyrosine kinase activity in two breast cancer lines - MCF-7 and MDA-MB-231.

Urszula Lewandowska, Agnieszka Lachowicz-Ochędalska, Kamila Domińska, Dominika Kaszewska, Elżbieta Rębas

Department of Medical Enzymology, Medical University, Łódź, Poland.

Endokrynologia Polska 2011

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Angiotensin II (AngII), a peptide that regulates the water-electrolytic balance and blood pressure, is also known to influence cell proliferation. It can either induce cell growth, when binding to angiotensin type-I receptor, or trigger growth inhibition via angiotensin type-II receptor. AngII stimulates proliferation of some normal and tumour cell lines, e.g. pituitary, adrenal glands and breast cancer. The aim of this study was to evaluate possible AngII effect on the growth of two breast cancer cell lines - hormone-dependent MCF-7 and hormone-independent MDA-MB-231. We measured tyrosine kinase activity as a potential proliferation marker. We also estimated the influence of 17b-oestradiolon AngII-induced changes. In the MDA-MB-231 line, AngII radically slowed the activity of tyrosine kinases and 17b-oestradiol only at a concentration of 10⁻⁶ M, while it enhanced the effect of angiotensin II at a concentration of 10⁻⁹ M. In MCF-7, Ang II had a strong inhibitory effect in the presence of oestradiol (10⁻⁶ M). Oestradiol alone decreased the activity of examined enzymes in both cell lines. AngII receptor type 1 was found in both studied lines, but type 2 only in MDA-MB-231. Our results show that AngII can modulate tyrosine kinase activity in breast tumour cell lines.

Citation

Urszula Lewandowska, Agnieszka Lachowicz-Ochędalska, Kamila Domińska, Dominika Kaszewska, Elżbieta Rębas. Angiotensin II as a factor modulating protein tyrosine kinase activity in two breast cancer lines - MCF-7 and MDA-MB-231. Endokrynologia Polska. 2011;62(2):151-8