Transgenic production of arachidonic acid in oilseeds.

James R Petrie, Pushkar Shrestha, Srinivas Belide, Maged P Mansour, Qing Liu, James Horne, Peter D Nichols, Surinder P Singh

Food Futures National Research Flagship, CSIRO Plant Industry, PO Box 1600, Canberra, ACT, 2601, Australia.

Transgenic research 2012 Feb

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We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate. We also demonstrate that the arachidonic acid is naturally enriched at the sn2 position in triacylglycerol. This is the first report of ARA production by the Δ9-elongase pathway in an oilseed.

Citation

James R Petrie, Pushkar Shrestha, Srinivas Belide, Maged P Mansour, Qing Liu, James Horne, Peter D Nichols, Surinder P Singh. Transgenic production of arachidonic acid in oilseeds. Transgenic research. 2012 Feb;21(1):139-47