Detection of glutathione in whole blood after stabilization with N-ethylmaleimide.

Daniela Giustarini, Isabella Dalle-Donne, Aldo Milzani, Ranieri Rossi

Department of Evolutionary Biology, Laboratory of Pharmacology and Toxicology, University of Siena, 53100 Siena, Italy. giustarini@unisi.it

Analytical biochemistry 2011 Aug 1

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The measurement of glutathione is a challenging task in that, during sample manipulation, a large percentage of this compound can be artificially oxidized. Here a high-performance liquid chromatography (HPLC) method to measure reduced glutathione in blood, based on the analysis of its conjugate with N-ethylmaleimide, has been developed and validated. The method is linear in the concentration range 0.1-2 mM, and the lower limit of quantification is 0.05 mM. Blood samples treated with N-ethylmaleimide are stable at -20°C for 90 days. This method has simplicity and rapidity as its main advantages; therefore, it is particularly suitable for large-scale clinical studies. Copyright © 2011 Elsevier Inc. All rights reserved.

Citation

Daniela Giustarini, Isabella Dalle-Donne, Aldo Milzani, Ranieri Rossi. Detection of glutathione in whole blood after stabilization with N-ethylmaleimide. Analytical biochemistry. 2011 Aug 1;415(1):81-3