Prediction of Michaelis-Menten constant of beta-glucosidases using nitrophenyl-beta-D-glucopyranoside as substrate.

State Key Laboratory of Nonfood Biomass Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi, 530007, China.

Protein and peptide letters 2011 Oct

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In this study, we attempted to use the neural network to model a quantitative structure-K(m) (Michaelis-Menten constant) relationship for beta-glucosidase, which is an important enzyme to cut the beta-bond linkage in glucose while K(m) is a very important parameter in enzymatic reactions. Eight feedforward backpropagation neural networks with different layers and neurons were applied for the development of predictive model, and twenty-five different features of amino acids were chosen as predictors one by one. The results show that the 20-1 feedforward backpropagation neural network can serve as a predictive model while the normalized polarizability index as well as the amino-acid distribution probability can serve as the predictors. This study threw lights on the possibility of predicting the K(m) in beta-glucosidases based on their amino-acid features.

Citation

Shaomin Yan, Guang Wu. Prediction of Michaelis-Menten constant of beta-glucosidases using nitrophenyl-beta-D-glucopyranoside as substrate. Protein and peptide letters. 2011 Oct;18(10):1053-7