Correlation Engine 2.0
Clear Search sequence regions

Numerous recent studies have suggested that the predicted cytosolic domains of G protein-coupled receptors represent a surface for association with proteins that may serve multiple roles in receptor localization, turnover, and signaling beyond the well-characterized interactions of these receptors with heterotrimeric G proteins. This Chapter describes two in vitro methods for ascertaining interactions between G protein-coupled receptors and various binding partners: gel overlay strategies and GST-fusion protein pull-downs.


Ashley E Brady, Yunjia Chen, Lee E Limbird, Qin Wang. Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs. Methods in molecular biology (Clifton, N.J.). 2011;746:347-55

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 21607867

View Full Text