Sanggenon C and O inhibit NO production, iNOS expression and NF-κB activation in LPS-induced RAW264.7 cells.

Nguyen Tien Dat, Phung Thi Xuan Binh, Le Thi Phuong Quynh, Hoang Thanh Huong, Chau Van Minh

Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam. ngtiend@hotmail.com

Immunopharmacology and immunotoxicology 2012 Feb

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The NO production through the iNOS induction by activation of nuclear factor (NF-κB) is known to involve in various inflammatory conditions. Sanggenon C and O, two Diels-Alder type adducts isolated from Morus alba, a plant has been used for the anti-inflammatory purpose in the Oriental medicine, were investigated for their effect on the NO production, iNOS expression and NF-κB activity. The inhibitory effects of sanggenon C and O on the NF-κB activity were investigated in LPS-stimulated RAW264.7 cells by SEAP reporter assay. The regulation of the iNOS expression and IκBα activation by two compounds was also evaluated by Western blot. Both compounds strongly inhibited NO production and NF-κB activation in a dose-dependent manner. The expression of the iNOS protein was also suppressed by treatment of the compounds (10 and 1 µM). Sanggenon O showed stronger inhibition than the diastereomer sanggenon C. Both compounds prevented the phosphorylation and degradation of IκBα protein. We demonstrated that sanggenon C and O inhibited NO production and iNOS expression by suppressing NF-κB activity and IκBα activation.

Citation

Nguyen Tien Dat, Phung Thi Xuan Binh, Le Thi Phuong Quynh, Hoang Thanh Huong, Chau Van Minh. Sanggenon C and O inhibit NO production, iNOS expression and NF-κB activation in LPS-induced RAW264.7 cells. Immunopharmacology and immunotoxicology. 2012 Feb;34(1):84-8