Amyloid of the Candida albicans Ure2p prion domain is infectious and has an in-register parallel β-sheet structure.

Abbi Engel, Frank Shewmaker, Herman K Edskes, Fred Dyda, Reed B Wickner

Biochemistry 2011 Jul 12

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Ure2p of Candida albicans (Ure2(albicans) or CaUre2p) can be a prion in Saccharomyces cerevisiae, but Ure2p of Candida glabrata (Ure2(glabrata)) cannot, even though the Ure2(glabrata) N-terminal domain is more similar to that of the S. cerevisiae Ure2p (Ure2(cerevisiae)) than Ure2(albicans) is. We show that the N-terminal N/Q-rich prion domain of Ure2(albicans) forms amyloid that is infectious, transmitting [URE3alb] to S. cerevisiae cells expressing only C. albicans Ure2p. Using solid-state nuclear magnetic resonance of selectively labeled C. albicans Ure2p(1-90), we show that this infectious amyloid has an in-register parallel β-sheet structure, like that of the S. cerevisiae Ure2p prion domain and other S. cerevisiae prion amyloids. In contrast, the N/Q-rich N-terminal domain of Ure2(glabrata) does not readily form amyloid, and that formed upon prolonged incubation is not infectious.

Citation

Abbi Engel, Frank Shewmaker, Herman K Edskes, Fred Dyda, Reed B Wickner. Amyloid of the Candida albicans Ure2p prion domain is infectious and has an in-register parallel β-sheet structure. Biochemistry. 2011 Jul 12;50(27):5971-8