HIF-1α ODD polypeptides increased the expression of HIF1 and VEGF in hypoxic rat cortical neuron.

Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang, China.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology 2011 Dec

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To investigate the effect of HIF-1α ODD polypeptides on the expression of HIF-1α and VEGF in primary rat cortical neuron in hypoxia. Primary cultured neurons were exposed to hypoxia for 2, 4, and 8 h. Tat-ODD fusion polypeptide was added before hypoxia in the experimental groups. The expression of HIF-1α and VEGF was detected by Western blot and real time PCR. The levels of HIF-1α and VEGF peaked at 4 h then declined in hypoxia. The expression of HIF-1α and VEGF was increased by tat-ODD fusion polypeptide. tat-ODD fusion polypeptide might improve HIF1 adaptation to hypoxia by increased expression of HIF-1α and VEGF.

Citation

Xuemin Liu, Juan Du, Qi Xi. HIF-1α ODD polypeptides increased the expression of HIF1 and VEGF in hypoxic rat cortical neuron. Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology. 2011 Dec;32(6):1029-33