Transplacental genotoxicity evaluation of cypermethrin using alkaline comet assay.

Y V Murkunde, T N Sathya, N Subashini, P Balakrishna Murthy

International Institute of Biotechnology and Toxicology, Kanchipuram district, Padappai, India.

Human & experimental toxicology 2012 Feb

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Transplacental genotoxic effect of cypermethrin technical was investigated. Three doses (25, 50 and 75 mg/kg body weight) were administered to groups of pregnant Wistar rats during 6-15 days of gestation. Animals were killed on gestation day 20. Fetal blood and liver samples were evaluated for DNA damage using alkaline comet assay. A marginal increase in the mean percentage of DNA damage was recorded in both blood and liver samples of fetuses from cypermethrin-treated dams, but the values were not statistically significant. No skeletal or visceral fetal abnormalities were recorded in treated groups. Nevertheless, the results lead to an understanding that transplacental exposure to cypermethrin can induce low levels of DNA damage in fetuses. This observation could be an explanation for the teratogenic effect exhibited by this chemical in many other studies. The results indicate that cypermethrin may be transplacentally genotoxic. The authors propose more detailed investigations for validating the current findings.

Citation

Y V Murkunde, T N Sathya, N Subashini, P Balakrishna Murthy. Transplacental genotoxicity evaluation of cypermethrin using alkaline comet assay. Human & experimental toxicology. 2012 Feb;31(2):185-92