Expression and structural characterization of human translocase of inner membrane of mitochondria Tim50.

Yongqiang Zhang, Yun Xu, Qing Zhao, Zhina Ji, Qiang Li, Shu Jie Li

The Key Laboratory of Bioactive Materials, Ministry of Education, School of Physics Science, Nankai University, Tianjin 300071, PR China.

Protein expression and purification 2011 Nov

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The preprotein translocase of the inner membrane of mitochondria (TIM23 complex) is the main entry gate for proteins of the matrix and the inner membrane. Tim50 is a major receptor in TIM23 complex, which spans the inner membrane with a single transmembrane segment and exposes a large hydrophilic domain in the intermembrane space. In this study, we expressed and purified the intermembrane space (IMS) domain of human Tim50 (Tim50(IMS)), and investigated its structural characteristics and assembly behaviors. The far-UV CD spectra of Tim50(IMS) in native and denatured states revealed that the protein has a significantly folded secondary structure consisted of α-helixes and β-sheets. Size exclusion chromatography showed that Tim50(IMS) is a monomer. Furthermore, the results showed, by intrinsic fluorescence, ANS binding, fluorescence anisotropy and fluorescence quenching, that Tim50(IMS) forms a compact structure in the range of pH 8.0-5.0; and it is more compact at pH 8.0 than pH 7.0; when pH decreases below 5.0, the protein is gradually denatured. Copyright © 2011 Elsevier Inc. All rights reserved.

Citation

Yongqiang Zhang, Yun Xu, Qing Zhao, Zhina Ji, Qiang Li, Shu Jie Li. Expression and structural characterization of human translocase of inner membrane of mitochondria Tim50. Protein expression and purification. 2011 Nov;80(1):130-7