Using superfolder green fluorescent protein for periplasmic protein localization studies.

Department of Microbiology and Immunobiology, Harvard Medical School, Armenise Building, Room 302A, 200 Longwood Avenue, Boston, MA 02115, USA.

Journal of bacteriology 2011 Sep

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Studies investigating the subcellular localization of periplasmic proteins have been hampered by problems with the export of green fluorescent protein (GFP). Here we show that a superfolding variant of GFP (sfGFP) is fluorescent following Sec-mediated transport and works best when the cotranslational branch of the pathway is employed. Copyright © 2011, American Society for Microbiology. All Rights Reserved.

Citation

Thuy Dinh, Thomas G Bernhardt. Using superfolder green fluorescent protein for periplasmic protein localization studies. Journal of bacteriology. 2011 Sep;193(18):4984-7