A role for CSLD3 during cell-wall synthesis in apical plasma membranes of tip-growing root-hair cells.

Sungjin Park, Amy L Szumlanski, Fangwei Gu, Feng Guo, Erik Nielsen

Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA.

Nature cell biology 2011 Aug

filter terms:

In plants, cell shape is defined by the cell wall, and changes in cell shape and size are dictated by modification of existing cell walls and deposition of newly synthesized cell-wall material. In root hairs, expansion occurs by a process called tip growth, which is shared by root hairs, pollen tubes and fungal hyphae. We show that cellulose-like polysaccharides are present in root-hair tips, and de novo synthesis of these polysaccharides is required for tip growth. We also find that eYFP-CSLD3 proteins, but not CESA cellulose synthases, localize to a polarized plasma-membrane domain in root hairs. Using biochemical methods and genetic complementation of a csld3 mutant with a chimaeric CSLD3 protein containing a CESA6 catalytic domain, we provide evidence that CSLD3 represents a distinct (1→4)-β-glucan synthase activity in apical plasma membranes during tip growth in root-hair cells.

Citation

Sungjin Park, Amy L Szumlanski, Fangwei Gu, Feng Guo, Erik Nielsen. A role for CSLD3 during cell-wall synthesis in apical plasma membranes of tip-growing root-hair cells. Nature cell biology. 2011 Aug;13(8):973-80