Assessment of two immobilized lipases activity and stability to low temperatures in organic solvents under ultrasound-assisted irradiation.

Luciane Batistella, Mara K Ustra, Aline Richetti, Sibele B C Pergher, Helen Treichel, J V Oliveira, Lindomar Lerin, Débora de Oliveira

Departamento de Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões, URI—Campus de Erechim, Av. 7 de Setembro, Erechim, RS, Brazil.

Bioprocess and biosystems engineering 2012 Mar

filter terms:

Both stability and catalytic activity of two commercial immobilized lipases were investigated in the presence of different organic solvents in ultrasound-assisted system. In a general way, for Novozym 435, the use of ethanol as solvent led to a loss of activity of 35% after 10 h of contact. The use of iso-octane conducted to a gradual increase in lipase activity in relation to the contact time, reaching a maximum value of relative activity of 126%. For Lipozyme RM IM, after 5 h of exposure, the enzyme presented no residual activity when ethanol was used as solvent. The solvents tert-butanol and iso-octane showed an enhancement of about 20 and 17% in the enzyme activity in 6 h of exposure, respectively. Novozym 435 and Lipozyme IM presented high stability to storage after treatment under ultrasound-assisted system using n-hexane and tert-butanol as solvents.

Citation

Luciane Batistella, Mara K Ustra, Aline Richetti, Sibele B C Pergher, Helen Treichel, J V Oliveira, Lindomar Lerin, Débora de Oliveira. Assessment of two immobilized lipases activity and stability to low temperatures in organic solvents under ultrasound-assisted irradiation. Bioprocess and biosystems engineering. 2012 Mar;35(3):351-8