Histopathological and proteomic analysis of hepatic tissue from adult male zebrafish exposed to 17β-estradiol.

17β-Estradiol (E2) is known to contribute significantly a large extent to the estrogenicity in aquatic system. In the present study, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MALDI-TOF-TOF MS), combined with histopathological analysis, was used to screen hepatic responses in adult male zebrafish. Eight proteins were found to be up-regulated more than 2-fold, whereas five protein spots were down-regulated more than 2-fold after 1nM E2 treatment for 14 days, which had caused histological effects in zebrafish livers. These differentially expressed proteins accounted for a variety of cellular biological processes, such as response to oxidative stress, cell surface receptor-linked signal transduction, oxidation-reduction and cellular calcium ion homeostasis. The results demonstrated that E2 comprehensively influenced a variety of cellular and biological processes in zebrafish. Moreover, the proteomic responses elicited in zebrafish allow us to better understand the underlying mechanisms of endocrine-disrupting chemicals (EDCs)-induced toxicity fully. Copyright © 2009 Elsevier B.V. All rights reserved.

Citation

Yuanxiang Jin, Xiangxiang Zhang, Dezhao Lu, Liwei Sun, Haifeng Qian, Weiping Liu, Zhengwei Fu. Histopathological and proteomic analysis of hepatic tissue from adult male zebrafish exposed to 17β-estradiol. Environmental toxicology and pharmacology. 2010 Jan;29(1):91-5

PMID: 21787588

search → result