Phospholipid scramblase 1 mediates hepatitis C virus entry into host cells.

Qiaoling Gong, Min Cheng, Hui Chen, Xiuying Liu, Youhui Si, Yang Yang, Yanzhi Yuan, Chaozhi Jin, Wei Yang, Fuchu He, Jian Wang

Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

FEBS letters 2011 Sep 2

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Hepatitis C virus (HCV) infects human hepatocytes through several host factors. However, other prerequisite factors for viral entry remain to be identified. Using a yeast two-hybrid screen, we found that human phospholipid scramblase 1 interacts with HCV envelope proteins E1 and E2. These physical interactions were confirmed by co-immunoprecipitation and GST pull-down assays. Knocking down the expression of PLSCR1 inhibited the entry of HCV pseudoparticles. Moreover, PLSCR1 was required for the initial attachment of HCV onto hepatoma cells, where it specifically interacted with entry factor OCLN. We show that PLSCR1 is a novel attachment factor for HCV entry. Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Citation

Qiaoling Gong, Min Cheng, Hui Chen, Xiuying Liu, Youhui Si, Yang Yang, Yanzhi Yuan, Chaozhi Jin, Wei Yang, Fuchu He, Jian Wang. Phospholipid scramblase 1 mediates hepatitis C virus entry into host cells. FEBS letters. 2011 Sep 2;585(17):2647-52