Suppression of inflammation by recombinant Salmonella typhimurium harboring CCL22 microRNA.

Won Suck Yoon, Seung Rel Ryu, Seung Seok Lee, Yang Seok Chae, Eun Jae Kim, Ji Hyun Choi, Sejin Oh, Se Ho Park, Ji Tae Choung, Young Yoo, Yong Keun Park

Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University, Seoul, Korea. biokorea@korea.ac.kr

DNA and cell biology 2012 Mar

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Atopic dermatitis (AD) is an inflammatory, chronically relapsing, puritic skin disorder. These syndromes result from multifactorial inheritance, with interaction between genetic and environmental factors. In particular, the macrophage-derived chemokine CCL22 is directly implicated in skin inflammatory reactions and its levels are significantly elevated in serum and correlated with disease severity in AD. We tested the suppression of the CCL22 gene by microRNA (miRNA) and observed the effects in mice with inflammation similar to AD. We used Salmonella as a vector to deliver miRNA. The recombinant strain of Salmonella typhimurium expressing CCL22 miRNA (ST-miRCCL22) was prepared for in vivo knockdown of CCL22. ST-miRCCL22 was orally inoculated into mice and the CCL22 gene suppressed with CCL22 miRNA in the activated lymphocytes. IgE and interleukin-4 were inhibited and interferon-γ was induced after treatments with ST-miRCCL22 and CCL22 was suppressed. Further, Th17 cells were suppressed in the atopic mice treated with ST-miRCCL22. These results suggested that suppression of the CCL22 gene using Salmonella induced anti-inflammatory effects.

Citation

Won Suck Yoon, Seung Rel Ryu, Seung Seok Lee, Yang Seok Chae, Eun Jae Kim, Ji Hyun Choi, Sejin Oh, Se Ho Park, Ji Tae Choung, Young Yoo, Yong Keun Park. Suppression of inflammation by recombinant Salmonella typhimurium harboring CCL22 microRNA. DNA and cell biology. 2012 Mar;31(3):290-7