Checkpoints in adenoviral production: cross-contamination and E1A.

Dagmar J Haeussler, Alicia M Evangelista, Joseph R Burgoyne, Richard A Cohen, Markus M Bachschmid, David R Pimental

Vascular Biology Section, Boston University Medical Center, Boston, Massachusetts, United States of America.

PloS one 2011

filter terms:

Adenoviruses are widely used for overexpressing proteins in primary mammalian cells. Incorporation of the early viral gene, E1A, or viral cross-contamination can occur during amplification, and identification of these products is crucial as the transcription of unwanted genetic material can impact cell function and compromise data interpretation. Here we report methods for evaluation of contaminating adenovirus and E1 viral DNA.

Citation

Dagmar J Haeussler, Alicia M Evangelista, Joseph R Burgoyne, Richard A Cohen, Markus M Bachschmid, David R Pimental. Checkpoints in adenoviral production: cross-contamination and E1A. PloS one. 2011;6(8):e23160