MicroRNA-132 targets HB-EGF upon IgE-mediated activation in murine and human mast cells.

Viktor Molnár, Barbara Érsek, Zoltán Wiener, Zsófia Tömböl, Péter M Szabó, Péter Igaz, András Falus

Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary. molvik.dgci@gmail.com

Cellular and molecular life sciences : CMLS 2012 Mar

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MicroRNAs provide an additional layer in the regulation of gene expression acting as repressors with several targets at the posttranscriptional level. This study describes microRNA expression patterns during differentiation and activation of mast cells. The expression levels of 567 different mouse miRNAs were compared by microarray between c-Kit+ committed progenitors, mucosal mast cells, resting and IgE-crosslinked BMMCs in vitro. The strongest upregulation of miR-132 upon IgE-mediated activation was validated in human cord blood-derived mast cells as well. HB-EGF growth factor also upregulated upon activation and was ranked high by more prediction algorithms. Co-transfection of miR-132 mimicking precursor and the 3'UTR of human Hbegf-containing luciferase vector proves that the predicted binding site is functional. In line with this, neutralization of miR-132 by anti-miR inhibitor leads to sustained production of HB-EGF protein in activated mast cells. Our data provide a novel example for negative regulation of a growth factor by an upregulated miRNA. © Springer Basel AG 2011

Citation

Viktor Molnár, Barbara Érsek, Zoltán Wiener, Zsófia Tömböl, Péter M Szabó, Péter Igaz, András Falus. MicroRNA-132 targets HB-EGF upon IgE-mediated activation in murine and human mast cells. Cellular and molecular life sciences : CMLS. 2012 Mar;69(5):793-808