Streptomyces ghanaensis pleiotropic regulatory gene wblA(gh) influences morphogenesis and moenomycin production.

Mariia Rabyk, Bohdan Ostash, Yurij Rebets, Suzanne Walker, Victor Fedorenko

Department of Genetic and Biotechnology, Ivan Franko National University of Lviv, Grushevskogo St. 4, Lviv 79005, Ukraine. Availl@i.ua

Biotechnology letters 2011 Dec

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The wblA(gh) gene, encoding a homologue of the WhiB-family of proteins, was identified in the sequenced genome of moenomycin producer Streptomyces ghanaensis. Deletion of the gene blocked aerial mycelium sporulation and caused a 230% increase in moenomycins production. S. ghanaensis overexpressing SSFG-01620: a homologue of extracellular protease inhibitor SCO0762, whose expression in Streptomyces coelicolor is down-regulated by wblA: showed deficiencies in sporulation similar to that of wblA(gh) knockout strain. The wblA(gh) gene of S. ghanaensis appears to play a negative role in the control of moenomycin biosynthesis and is essential for sporulation. © Springer Science+Business Media B.V. 2011

Citation

Mariia Rabyk, Bohdan Ostash, Yurij Rebets, Suzanne Walker, Victor Fedorenko. Streptomyces ghanaensis pleiotropic regulatory gene wblA(gh) influences morphogenesis and moenomycin production. Biotechnology letters. 2011 Dec;33(12):2481-6