The C2H2 zinc finger transcription factors are likely targets for Ni(II) toxicity.

Ewa Kurowska, Joanna Sasin-Kurowska, Arkadiusz Bonna, Marcin Grynberg, Jarosław Poznański, Lukasz Knizewski, Krzysztof Ginalski, Wojciech Bal

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland.

Metallomics : integrated biometal science 2011 Nov

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Ni(II) ions are able to hydrolyze Naa-(Ser/Thr) peptide bonds in Naa-(Ser/Thr)-Xaa-His-Zaa sequences. We found that various human transcription factors contain such nickel hydrolytic patterns within C2H2 zinc finger (ZF) domains. We demonstrated the hydrolysis on two peptide models, the 3rd ZF of the Sp1 transcription factor and the 1st ZF of the ZNF302 transcription factor. The experimentally studied reaction rates indicate that the hydrolysis reaction is likely to be an element of intracellular nickel toxicity.

Citation

Ewa Kurowska, Joanna Sasin-Kurowska, Arkadiusz Bonna, Marcin Grynberg, Jarosław Poznański, Lukasz Knizewski, Krzysztof Ginalski, Wojciech Bal. The C2H2 zinc finger transcription factors are likely targets for Ni(II) toxicity. Metallomics : integrated biometal science. 2011 Nov;3(11):1227-31