Quantification of hemoglobin Constant Spring in heterozygote and homozygote by a capillary electrophoresis method.

S Pornprasert, S Panyasai, J Waneesorn, K Kongthai, P Singboottra

Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang-Mai University, Chiang-Mai, Thailand. sakornmi001@gmail.com

International journal of laboratory hematology 2012 Apr

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Capillary electrophoresis (CE) is a high-resolution method for detection of hemoglobin Constant Spring (Hb CS). The levels of Hb CS quantified by CE were compared among three groups of samples including heterozygote and homozygote of Hb CS as well as Hb H-CS disease classified by DNA molecular diagnosis. The mean corpuscular volume (MCV) of red blood cells was also analyzed among these three groups. Mean ± SD of Hb CS level of the homozygote was not significantly different from that of the Hb H-CS disease (1.9 ± 1.8 vs. 2.8 ± 1.3, P = 0.13), but it was significantly higher than that of the heterozygote (1.9 ± 1.8 vs. 0.4 ± 0.2, P = 0.007). The MCV <70 fL was found in Hb H-CS disease only. CE is the preferable method for screening of heterozygote and homozygote of Hb CS. Moreover, in conjunction with a lower MCV (<70 fL), this approach provided a high resolution to identify Hb H-CS disease. © 2011 Blackwell Publishing Ltd.

Citation

S Pornprasert, S Panyasai, J Waneesorn, K Kongthai, P Singboottra. Quantification of hemoglobin Constant Spring in heterozygote and homozygote by a capillary electrophoresis method. International journal of laboratory hematology. 2012 Apr;34(2):143-7