Utilization of glycosyltransferases for the synthesis of a densely packed hyperbranched polysaccharide brush coating as artificial glycocalyx.

Jeroen van der Vlist, Iris Schönen, Katja Loos

Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

Biomacromolecules 2011 Oct 10

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Densely packed polysaccharide brushes consisting of α-D-glucose residues were grafted from modified silicon substrates. Potato phosphorylase was herein used to grow linear polysaccharide chains from silicon tethered maltoheptaose oligosaccharides using glucose-1-phosphate as donor substrate. The combined use of potato phosphorylase and Deinococcusgeothermalis branching enzyme resulted in a hyperbranched brush coating as the latter one redistributes short oligosaccharides from the α(1-4)-linked position to the α (1-6)-linked position in the polysaccharide brush. The obtained grafting density of the brushes was estimated on 1.89 nm(-2) while the thickness was measured with ellipsometric techniques and determined to be between 12.2 and 20.2 nm.

Citation

Jeroen van der Vlist, Iris Schönen, Katja Loos. Utilization of glycosyltransferases for the synthesis of a densely packed hyperbranched polysaccharide brush coating as artificial glycocalyx. Biomacromolecules. 2011 Oct 10;12(10):3728-32