A colorimetric assay of protein kinase activity based on peptide-induced coagulation of gold nanorods.

Hirotaro Kitazaki, Takeshi Mori, Jeong-Hun Kang, Takuro Niidome, Masaharu Murata, Makoto Hashizume, Yoshiki Katayama

Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan.

Colloids and surfaces. B, Biointerfaces 2012 Nov 1

filter terms:

We succeeded in applying gold nanorods (NRs) for the detection of protein kinase (PK) activity based on a substrate-induced coagulation mechanism. Because NRs have cationic surface charges due to surface-adsorbed cetyltrimethylammonium bromide, phosphorylated substrate peptides, which gain augmented anionic charges by phosphorylation, induce coagulation of the NRs, resulting in a red-to-blue color change of the corresponding NR dispersion. A large absorption coefficient of the NRs at the near-IR region enabled clear-cut detection of the peptide-induced coagulation. IC(50) values of several inhibitors of c-Src determined by the present assay are consistent with reported values. Copyright © 2011 Elsevier B.V. All rights reserved.

Citation

Hirotaro Kitazaki, Takeshi Mori, Jeong-Hun Kang, Takuro Niidome, Masaharu Murata, Makoto Hashizume, Yoshiki Katayama. A colorimetric assay of protein kinase activity based on peptide-induced coagulation of gold nanorods. Colloids and surfaces. B, Biointerfaces. 2012 Nov 1;99:7-11