A discussion of molecular biology methods for protein engineering.

Alexander Zawaira, Anil Pooran, Samantha Barichievy, Denis Chopera

Gene Expression and Biophysics Group, Synthetic Biology-ERA, CSIR Biosciences, Brummeria, Pretoria 0001, South Africa. azawaira@gmail.com

Molecular biotechnology 2012 May

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A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. We discuss the basic principles of these methods in a repertoire that may be used to achieve the elemental steps in protein engineering. These include site-directed, deletion and insertion mutagenesis. We provide detailed case studies, drawn from our own experiences, packaged together with conceptual discussions and include an analysis of the techniques presented with regards to their uses in protein engineering.

Citation

Alexander Zawaira, Anil Pooran, Samantha Barichievy, Denis Chopera. A discussion of molecular biology methods for protein engineering. Molecular biotechnology. 2012 May;51(1):67-102