Neurofilament immune fluorescence staining: a new method for observing the morphology of the motor endplate].

Yu-hui Kou, Tian-bing Wang, Pei-xun Zhang, Xiao-feng Yin, Na Han, Jing Wang, Hong-bo Zhang, Dian-ying Zhang, Bao-guo Jiang

Department of Trauma and Orthopeadics, Peking University People's Hospital, Beijing 100044, China.

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences 2011 Oct 18

filter terms:

To investigate the effect of neurofilament (NF) immune fluorescence staining on observing morphology of the motor endplate. Six SPF rats were used and their bilateral soleus muscles harvested under anesthesia. Then the samples were fixed with polyphosphate formaldehyde, and made frozen sections. Finally the neurofilament immunofluorescence staining was performed. The morphology of the motor endplate was observed under fluorescence microscope and laser scanning confocal fluorescence microscope. The claw-shape motor endplate was seen clearly under the fluorescent microscope and laser scanning confocal fluorescence microscope, and the images observed used to reconstruct the three-dimensional structure of motor endplate. Neurofilament immune fluorescence staining is a useful method for the morphology study of the motor endplate.

Citation

Yu-hui Kou, Tian-bing Wang, Pei-xun Zhang, Xiao-feng Yin, Na Han, Jing Wang, Hong-bo Zhang, Dian-ying Zhang, Bao-guo Jiang. Neurofilament immune fluorescence staining: a new method for observing the morphology of the motor endplate]. Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences. 2011 Oct 18;43(5):774-6