N'Cho Christophe Amin, Marie-Dominique Blanchin, Michèle Aké, Huguette Fabre
Laboratoire de Chimie Analytique, Contrôle physico-chimique des médicaments, Institut des Biomolécules Max Mousseron, UMR 5247, Faculté de Pharmacie, BP 14491, 34093 Montpelier, France.
Journal of pharmaceutical and biomedical analysis 2012 Jan 25A novel, simple and rapid capillary zone electrophoresis method with UV detection has been developed for the simultaneous determination of pyrimethamine and sulfadoxine in tablet formulations. The compounds are separated in 6 min in a fused silica capillary, 30 cm long (20 cm to detector)× 50 μm using a 100 mM phosphate buffer pH 7.2 as background electrolyte, a 330 V cm(-1) electric field and a detection wavelength of 214 nm. Analysis of different tablet formulations has shown a good agreement with the liquid chromatography method described in the United States Pharmacopoeia. Main advantages of the CZE method are the rapid set-up of instrumentation and capillary equilibration, short analysis time and low running cost. Copyright © 2011 Elsevier B.V. All rights reserved.
N'Cho Christophe Amin, Marie-Dominique Blanchin, Michèle Aké, Huguette Fabre. Capillary zone electrophoresis as a potential technique for the simultaneous determination of sulfadoxine and pyrimethamine in tablet formulations. Journal of pharmaceutical and biomedical analysis. 2012 Jan 25;58:168-71
PMID: 22014652
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