Yingzi Fu, Qiao Chen, Juan Zhou, Qian Han, Yonghua Wang
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China. fyzc@swu.edu.cn
Analytical biochemistry 2012 Feb 1A new chiral biosensor has been fabricated by immobilizing γ-globulin on gold nanoparticles modified glassy carbon electrodes, which could recognize and detect mandelic acid (MA) enantiomers. Differential pulse voltammetry, quartz crystal microbalance, ultraviolet-visible spectroscopy, and atomic force microscopy were used to characterize the enantioselectivity. The results exhibited that γ-globulin modified electrode could enantioselectively recognize MA enantiomers, and larger response signals were obtained from R-MA. The factors influencing the performance of the resulting biosensor were investigated. The enantiomeric composition of R- and S-MA enantiomer mixtures could be determined by measuring the current responses of the sample. The developed electrodes have the advantages of simple preparation, good stability, and rapid detection. Copyright © 2011 Elsevier Inc. All rights reserved.
Yingzi Fu, Qiao Chen, Juan Zhou, Qian Han, Yonghua Wang. Enantioselective recognition of mandelic acid based on γ-globulin modified glassy carbon electrode. Analytical biochemistry. 2012 Feb 1;421(1):103-7
PMID: 22056409
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