Functional analysis of the cyclopiazonic acid biosynthesis gene cluster in Aspergillus oryzae RIB 40.

Yasutomo Shinohara, Masafumi Tokuoka, Yasuji Koyama

Noda Institute for Scientific Research, Noda, Chiba, Japan. yshinohara@mail.kikkoman.co.jp

Bioscience, biotechnology, and biochemistry 2011

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The cyclopiazonic acid (CPA) nonproducing strain, Aspergillus oryzae RIB 40, does not biosynthesize cyclo-acetoacetyl-L-tryptophan (cAATrp) due to a truncation in the responsible PKS-NRPS gene. We found that RIB 40 converted cAATrp to 2-oxocyclopiazonic acid, the final product of CPA biosynthesis in A. oryzae. This indicates that the CPA biosynthesis gene cluster, except for the PKS-NRPS gene, is functional in RIB 40.

Citation

Yasutomo Shinohara, Masafumi Tokuoka, Yasuji Koyama. Functional analysis of the cyclopiazonic acid biosynthesis gene cluster in Aspergillus oryzae RIB 40. Bioscience, biotechnology, and biochemistry. 2011;75(11):2249-52