Quantitative determination of peimisin and total alkaloids in Fritillaria taipaiensis of different growing stage].

To study the content of peimisine and total alkaloids in Fritllaria taipaiensis of different growing stage. High performance liquid chromatography coupled with evaporative light scattering detector was employed, and peimisine was separated and quantified upon an Agilent Extend C18 (250 mm x 4.0 mm, 5 microm), using acetonitrile-water-diethylamine as the mobile phase at the flow rate of 1.0 mL min(-1); UV spectrophotometry was used for the determination of total alkaloids. During from two to three years period, the content of peimisine in F. taipaiensis increased with growing stage, from four to six years, it decreased with growing stage; From two to four years, the content of total alkaloids in F. taipaiensis increased with growing stage, from five to six years, it decreased with growing stage. It is suitable to harvest F. taipaienssi of four years growing stage.

Citation

Huai-yu Wang, Peng Ma, Rui Peng. Quantitative determination of peimisin and total alkaloids in Fritillaria taipaiensis of different growing stage]. Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials. 2011 Jul;34(7):1034-7

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PMID: 22066394

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