Measurement of pyrene in the gills of exposed fish using synchronous fluorescence spectroscopy.

A synchronous fluorescence spectroscopy (SFS) method was developed for determination of pyrene in the gills of exposed fish. The wavelength differences (Δλ) of 50 nm was maintained between excitation and emission wavelengths and it was found to be suitable for the effective determination of pyrene in fish gills; the peak were observed at λ(ex) 334.5 nm. Linear relationships between SFS intensity and the concentration of pyrene in n-hexane solution were established. It was demonstrated that the SFS method was effective, simple, and less expensive, providing an attractive alternative for the rapid analysis of pyrene in fish gills. Copyright © 2011 Elsevier Ltd. All rights reserved.

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Xianli Liu, Jinqiu Jing, Shengfang Li, Guodong Zhang, Tao Zou, Xianyou Xia, Weidong Huang. Measurement of pyrene in the gills of exposed fish using synchronous fluorescence spectroscopy. Chemosphere. 2012 Jan;86(2):198-201

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PMID: 22071375

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