Effectiveness of calcium hydroxide-based intracanal medicaments against Enterococcus faecalis.

R K P Lima, J M Guerreiro-Tanomaru, N B Faria-Júnior, M Tanomaru-Filho

Department of Restorative Dentistry, Araraquara Dental School, UNESP - Univ Estadual Paulista, Araraquara, Brazil.

International endodontic journal 2012 Apr

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To evaluate the antimicrobial efficacy of calcium hydroxide-based intracanal medicaments against Enterococcus faecalis. One hundred and six single-rooted human teeth were inoculated with E. faecalis (ATCC 29212) and incubated in an microaerophilic environment for 21 days. Teeth were divided according to the medicaments and period: G1 - Calen (7 days); G2 - Calen (14 days); G3 - Calen/camphorated paramonochlorophenol (CMCP) (7 days); G4 - Calen/CMCP (14 days); G5 - Calen/0.4% chlorhexidine (CHX) (7 days); G6 - Calen/0.4% CHX (14 days); G7 - Calen/1% CHX (7 days); G8 - Calen/1% CHX (14 days); G9 - chemo-mechanical preparation with no medication and G10 - no chemo-mechanical preparation and no medication. Microbial samples were harvested immediately after removal of the intracanal dressing and 7 days later. Data were subjected to anova (P < 0.05). All medicaments were associated with a reduction in E. faecalis after removal of the dressing (post-medication harvest). However, all specimens were associated with an increase in CFU mL(-1) values after 7 days (final harvest). Calen/CMCP and Calen/CHX had the lowest CFU mL(-1) values (P < 0.05). In this laboratory study using extracted teeth all calcium hydroxide-based medicaments were able to significantly reduce the CFU mL(-1) values of E. faecalis in the root canal system. © 2011 International Endodontic Journal.

Citation

R K P Lima, J M Guerreiro-Tanomaru, N B Faria-Júnior, M Tanomaru-Filho. Effectiveness of calcium hydroxide-based intracanal medicaments against Enterococcus faecalis. International endodontic journal. 2012 Apr;45(4):311-6