Genome-wide analysis of mutagenesis bias and context sensitivity of N-methyl-N'-nitro-N-nitrosoguanidine (NTG).
Marc Harper, Christopher J Lee
University of California, Los Angeles, CA 90095-1570, USA. marcharper@ucla.edu
Mutation research 2012 Mar 1We have analyzed the mutation spectrum of N-methyl-N'-nitro-N-nitrosoguanidine (NTG) from a set of 4099 mutations identified from whole-genome sequencing of 32 E. coli strains mutagenized with NTG. These data permit precise measurement of NTG's bias for G/C to A/T transitions (96.6% of all mutations) and also show that NTG mutagenesis is strongly sensitive to context, favoring guanine residues preceded by purines by five-fold over those preceded by pyrimidines. These data give confident estimates for the GC bias and transition/transversion ratios of NTG mutagenesis, which could not be estimated confidently from previous, much smaller datasets. Published by Elsevier B.V.
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Marc Harper, Christopher J Lee. Genome-wide analysis of mutagenesis bias and context sensitivity of N-methyl-N'-nitro-N-nitrosoguanidine (NTG). Mutation research. 2012 Mar 1;731(1-2):64-7
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PMID: 22080106
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