Effects of pollen supply and quality on seed formation and maturation in Pinus densiflora.

To understand the detailed mechanisms underlying variations in seed productivity per cone, it is important to examine simultaneously the effects of two pollination mode components (pollen supply and quality) on two seed production processes (seed formation and maturation). We conducted artificial pollination experiments with four pollination treatments (selfing, polycross, no-pollination and open-pollination treatments) in each of two vertical crown layers (upper and lower) for 19 Pinus densiflora ramets. We measured formed seeds as a proportion of ovules (P(Form)), and filled seeds as a proportion of formed seeds (P(Fill)) per cone in each treatment and layer, and inferred the relative influences of pollination mode and resource availability on seed productivity. In the no-pollination treatment, no seeds were formed in any cones of all five ramets. The Generalized Linear Model showed that there were no significant differences in P(Form) both between selfing and polycross treatments and upper and lower layers. The mean P(Fill) values in the selfing treatment were significantly lower than those in the polycross treatment in both layers. The mean P(Fill)s of the two layers did not differ significantly in the selfing treatment, but did in the open-pollination and polycross treatments. The results show that pollen supply affects mainly seed formation, whereas pollen quality affects mainly seed maturation. Resource availability also affects mainly seed maturation, if pollen quality is higher than a certain threshold.

Citation

Masakazu G Iwaizumi, Makoto Takahashi. Effects of pollen supply and quality on seed formation and maturation in Pinus densiflora. Journal of plant research. 2012 Jul;125(4):517-25

PMID: 22080253

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