Bacterial whole-cell biosensor for glutamine with applications for quantifying and visualizing glutamine in plants.

Michael J Tessaro, Sameh S M Soliman, Manish N Raizada

Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada.

Applied and environmental microbiology 2012 Jan

filter terms:

A whole-cell biosensor for glutamine (GlnLux) was constructed by transforming an Escherichia coli glutamine (Gln) auxotroph with a constitutive lux reporter gene. Measurements of Gln in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman's r = 0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gln from whole plant organs.

Citation

Michael J Tessaro, Sameh S M Soliman, Manish N Raizada. Bacterial whole-cell biosensor for glutamine with applications for quantifying and visualizing glutamine in plants. Applied and environmental microbiology. 2012 Jan;78(2):604-6