Consequences of butylated hydroxytoluene in the freezing extender on post-thaw characteristics of stallion spermatozoa in vitro.

A Morillo-Rodríguez, B Macías-García, J A Tapia, C Ortega-Ferrusola, F J Peña

Laboratory of Equine Reproduction, Veterinary Teaching Hospital, Cáceres, Spain.

Andrologia 2012 May

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Ejaculates from six pure Spanish stallions were split, and one subsample frozen in a commercial extender supplemented with the lipid soluble antioxidant butylated hydroxytoluene (BHT), while the other subsample served as control. After at least 4 weeks of storage, samples were thawed and post-thaw sperm quality analysed: sperm motility and kinematics using a CASA system, membrane and acrosome integrity and mitochondrial membrane potential using flow cytometry. The outcome of cryopreservation varied significantly among stallions. However, the supplementation with 1 mm BHT had no significant effect on any of the sperm parameters evaluated post-thaw. © 2011 Blackwell Verlag GmbH.

Citation

A Morillo-Rodríguez, B Macías-García, J A Tapia, C Ortega-Ferrusola, F J Peña. Consequences of butylated hydroxytoluene in the freezing extender on post-thaw characteristics of stallion spermatozoa in vitro. Andrologia. 2012 May;44 Suppl 1:688-95