Dragon PolyA Spotter: predictor of poly(A) motifs within human genomic DNA sequences.

Manal Kalkatawi, Farania Rangkuti, Michael Schramm, Boris R Jankovic, Allan Kamau, Rajesh Chowdhary, John A C Archer, Vladimir B Bajic

Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.

Bioinformatics (Oxford, England) 2012 Jan 1

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Recognition of poly(A) signals in mRNA is relatively straightforward due to the presence of easily recognizable polyadenylic acid tail. However, the task of identifying poly(A) motifs in the primary genomic DNA sequence that correspond to poly(A) signals in mRNA is a far more challenging problem. Recognition of poly(A) signals is important for better gene annotation and understanding of the gene regulation mechanisms. In this work, we present one such poly(A) motif prediction method based on properties of human genomic DNA sequence surrounding a poly(A) motif. These properties include thermodynamic, physico-chemical and statistical characteristics. For predictions, we developed Artificial Neural Network and Random Forest models. These models are trained to recognize 12 most common poly(A) motifs in human DNA. Our predictors are available as a free web-based tool accessible at http://cbrc.kaust.edu.sa/dps. Compared with other reported predictors, our models achieve higher sensitivity and specificity and furthermore provide a consistent level of accuracy for 12 poly(A) motif variants. vladimir.bajic@kaust.edu.sa Supplementary data are available at Bioinformatics online.

Citation

Manal Kalkatawi, Farania Rangkuti, Michael Schramm, Boris R Jankovic, Allan Kamau, Rajesh Chowdhary, John A C Archer, Vladimir B Bajic. Dragon PolyA Spotter: predictor of poly(A) motifs within human genomic DNA sequences. Bioinformatics (Oxford, England). 2012 Jan 1;28(1):127-9