Identification and characterization of a xyloglucan-specific family 74 glycosyl hydrolase from Streptomyces coelicolor A3(2).

Bolormaa Enkhbaatar, Uyangaa Temuujin, Ju-Hyeon Lim, Won-Jae Chi, Yong-Keun Chang, Soon-Kwang Hong

Division of Bioscience and Bioinformatics, Myongji University, Yongin, South Korea.

Applied and environmental microbiology 2012 Jan

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The sco6545 gene of Streptomyces coelicolor A3(2) was nominated as a putative cellulase with 863 mature-form amino acids (90.58 kDa). We overexpressed and purified Sco6545 and demonstrated that the protein is not a cellulase but a xyloglucan-specific glycosyl hydrolase which cleaves xyloglucan at unbranched glucose residues.

Citation

Bolormaa Enkhbaatar, Uyangaa Temuujin, Ju-Hyeon Lim, Won-Jae Chi, Yong-Keun Chang, Soon-Kwang Hong. Identification and characterization of a xyloglucan-specific family 74 glycosyl hydrolase from Streptomyces coelicolor A3(2). Applied and environmental microbiology. 2012 Jan;78(2):607-11