Qualitative difference between "bulb" membranes and other vacuolar membranes.

Chieko Saito, Tomohiro Uemura, Chie Awai, Takashi Ueda, Hiroshi Abe, Akihiko Nakano

Molecular Membrane Biology Laboratory, RIKEN Advanced Science Institute, Wako, Japan. chiezo@riken.jp

Plant signaling & behavior 2011 Dec

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Bulb" is a mobile and complex structure appearing in vacuolar membrane of plant cell. We recently reported new fluorescent marker lines for bulbs and bulb-less mutants. We tried multicolor visualization of vacuolar membrane to show distinct segregation of bulb-positive protein (γTIP or AtVAM3) and bulb-negative protein (AtRab75). Unexpectedly, GFP-AtRab75 resulted to localize in bulb under the condition of co-expression with TagRFP-AtVAM3. The signal intensities of GFP-AtRab75 and TagRFP-AtVAM3 were quantified and compared. The result indicates that TagRFP-AtVAM3 is concentrated in bulb than GFP-AtRab75. © 2011 Landes Bioscience

Citation

Chieko Saito, Tomohiro Uemura, Chie Awai, Takashi Ueda, Hiroshi Abe, Akihiko Nakano. Qualitative difference between "bulb" membranes and other vacuolar membranes. Plant signaling & behavior. 2011 Dec;6(12):1914-7